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| Registros recuperados : 1 | |
| 1. |  | GIANNITTI, F.; FRAGA, M.; CAFFARENA, D.; SCHILD, C.; BANCHERO, G.; ARMIEN, AG.; TRAVERIA, G.; MARTHALER, D.; WELLS, S.; RIET-CORREA, F. Diagnosis and within-flock seroprevalence of ovine Johne`s disease caused by a sheep (type S) strain of Mycobacterium avium subsp. paratuberculosis in Uruguay. In: American Association of Veterinary Laboratory Diagnosticians (AAVLD), 59º; United States Animal Health Association (USAHA) Annual Meeting, Greensboro, North Carolina, Oct 13-19, 2016.| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 1 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
17/04/2024 |
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Actualizado : |
17/04/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. |
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Afiliación : |
ANA PAULA AREVALO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; SERGIO ANCHETA, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; KAREN PERELMUTER, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; ALEJANDRO RICCIARDI; MARIELA BOLLATI-FOGOLÍN, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; MARTINA CRISPO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay. |
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Título : |
3R's applied to in vivo biological activity of recombinant human erythropoietin assay. |
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Complemento del título : |
Technical Article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. |
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ISSN : |
2675-9225 |
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DOI : |
10.4322/2675-9225.00012023 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. --
Publisher: Brazilian Society for Laboratory Animal Science. |
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Contenido : |
ABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. |
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Palabras claves : |
3R’s; Biological activity; Erythropoietin; European Pharmacopeia; Mice; UNIDAD MIXTA PASTEUR + INIA. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://bmrtsbcaljournal.com/article/10.4322/2675-9225.00012023/pdf/bmrt-3-1-e00012023.pdf
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Marc : |
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022 $a2675-9225
024 7 $a10.4322/2675-9225.00012023$2DOI
100 1 $aAREVALO, A.P.
245 $a3R's applied to in vivo biological activity of recombinant human erythropoietin assay.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. -- Publisher: Brazilian Society for Laboratory Animal Science.
520 $aABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved.
653 $a3R’s
653 $aBiological activity
653 $aErythropoietin
653 $aEuropean Pharmacopeia
653 $aMice
653 $aUNIDAD MIXTA PASTEUR + INIA
700 1 $aBASIKA, T.
700 1 $aANCHETA, S.
700 1 $aPERELMUTER, K.
700 1 $aRICCIARDI, A.
700 1 $aBOLLATI-FOGOLÍN, M.
700 1 $aCRISPO, M.
773 $tBiological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS.
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